TNFSF11抗体

TNFSF11抗体

抗原
Tumor Necrosis Factor (Ligand) Superfamily, Member 11 (TNFSF11)
别名 TNFSF11, BTF3L1, HUMBTFB, OCIF, OPG, TNFRSF11B, CD254, ODF, OPGL, OPTB2, RANKL, TRANCE, hRANKL2, sOdf, Ly109l, Trance, Opg
适用
其他选择 Human
Human(115),Murine (103),Rat ((Rattus)) (32),Dog (Canine) (25),Bovine (13),Chicken (1),Pig (Porcine) (1)
宿主
其他选择 Rabbit
Rabbit(118),Rat (26),Mouse (25),Goat (7),Sheep (1)
克隆类型
多克隆
标记
其他选择 非结合性
Biotin(23),PE (10),FITC (4),HRP (4),Alexa Fluor 350 (2),Alexa Fluor 488 (2),Alexa Fluor 555 (2),Alexa Fluor 647 (2),Cy3 (2),Cy5 (2),Cy5.5 (2),Cy7 (2),APC (1),Alkaline Phosphatase (AP) (1)
应用范围
其他选择 Enzyme Immunoassay (EIA), Functional Studies (Func), Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)), Western Blotting (WB), Immunohistochemistry (Paraffin-embedded Sections) (IHC (p))
Western Blotting (WB)(126),ELISA (70),Flow Cytometry (FACS) (38),Immunohistochemistry (Paraffin-embedded Sections) (IHC (p)) (26),Immunohistochemistry (IHC) (23),Immunofluorescence (Paraffin-embedded Sections) (IF (p)) (20),Immunofluorescence (IF) (17),Immunocytochemistry (ICC) (12),Immunohistochemistry (Frozen Sections) (IHC (fro)) (10),Enzyme Immunoassay (EIA) (8),Immunoprecipitation (IP) (8),Blocking Antibody (Inhibition) (5),Immunoassay (IA) (3),Immunohistochemistry (Frozen Paraffin-embedded Sections) (IHC (frpe)) (3),Neutralization (Neut) (3),Blocking Reagent (BR) (1),Functional Studies (Func) (1)
[Pubmed] 2 references available
规格 0.1 mg
发货至 中国 (更改)
发货时间 29至35个工作日

TNFSF11抗体

产品细节

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产品细节

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免疫原 Highly pure (>98%) recombinant Human sRANKL.
特异性 This antibody reacts with soluble RANK Ligand.
纯化方法 Affinity Chromatography.

目标详细情况

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目标详细情况

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别名 CD254 / RANKL
背景 RANKL is a member of the tumor necrosis factor (TNF) cytokine family which is a ligand for osteoprotegerin and functions as a key factor for osteoclast differentiation and activation. There are three isoforms of RANKL. Human RANKL is a soluble 20 kDa polypeptide, comprising the TNF homologous region of RANKL (176 amino acid residues). This protein was shown to be a dentritic cell survival factor and is involved in the regulation of T cell dependent immune response. T cell activation was reported to induce expression of this gene and lead to an increase of osteoclastogenesis and bone loss. This protein was shown to activate antiapoptotic kinase AKT/PKB through a signaling complex involving SRC kinase and tumor necrosis factor receptor associated factor (TRAF) 6, which indicated that this protein may have a role in the regulation of cell apoptosis. RANKL deficient mice show severe osteoporesis and complete absence of osteoclasts as a result of lack of osteogenesis.Synonyms: ODF, OPGL, Osteoclast differentiation factor, Osteoprotegerin ligand, RANK Ligand, RANKL, Receptor activator of nuclear factor kappa B ligand, TNF-related activation-induced cytokine, TNFSF11, TRANCE, Tumor necrosis factor ligand superfamily member 11
基因ID 8600
NCBI Accession NP_003692
UniProt O14788

使用细节

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应用备注 Neutralization. Immunohistochemistry on Formalin-Fixed, Parrafin-embedded Sections: 0.25 μg/mL withan overnight incubation at 4 °C. Staining of Human metastatic carcinoma of lymph nodefrom breast tissue. An HRP-labeled polymer detection system was used with DABchromogen. Heat induced antigen retrieval with a pH 6.0 Sodium Citrate buffer isrecommended. Sandwich ELISA: To detect Human sRANKL by Sandwich ELISA (using 100 μL/well antibodysolution) a concentration of at least 0.5-2.0 μg/mL of this antibody is required. This antigenaffinity purified antibody, in conjunction with Biotinylated anti-Human sRANKL (PP1086B1or PP1086B2) as a Detection antibody, allows the detection of at least 0.2-0.4 ng/well ofrecombinant Human sRANKL. Western blot: To detect Human sRANKL by Western Blot analysis this antibody can be usedat a concentration of 0.1-0.2 μg/mL. Used in conjunction with compatible secondary
限制 For Research Use only

贮存及处理

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溶解方式 Restore in sterile water to a concentration of 0.1-1.0 mg/mL.
缓冲液 PBS, pH 7.2 without preservatives
储存液 Without preservative
注意事项 Avoid repeated freezing and thawing.
储存条件 4 °C/-20 °C
储存方法 Store the antibody prior to reconstitution at -20 °C. Following reconstitution the antibody can be stored at 2-8 °C for one month or at -20 °C for longer.

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