PFN4抗原;抑丝蛋白4(PFN4)重组蛋白多种属 是应用了重组DNA或重组RNA的技术从而获得的蛋白质。体外重组蛋白的生产主要包括四大系统:原核蛋白表达,哺乳动物细胞蛋白表达,酵母蛋白表达及昆虫细胞蛋白表达。生产的蛋白在活性和应用方法方面均有所不同。根据自身的下游运用选择合适的蛋白表达系统,提高表达成功率。
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 90% |
分子量 | 17.6kDa | 货号 | XY94006RPHu01 |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB; ELISA; IP. | 应用领域 | 生物产业 |
Recombinant Profilin 4 (PFN4)
抑丝蛋白4(PFN4)重组蛋白
[ PROPERTIES ]
Residues: Met1~Arg125
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q8NHR9
Host: E. coli
Subcellular Location: Cytoplasm, cytoskeleton
Purity: >90%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 7.7
Predicted Molecular Mass: 17.6kDa
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
The sequence of the target protein is listed below.
MSHLQSLLLD TLLGTKHVDS AALIKIQERS LCVASPGFNV TPSDVRTLVN GFAKNPLQARREGLYFKGKD YRCVRADEYS LYAKNENTGV VVVKTHLYLL VATYTEGMYP SICVEATESLGDYLR
[ IDENTIFICATION ]
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