NOTCH2抗原；Notch同源物2重组蛋白多种属

Recombinant Notch Homolog 2 (NOTCH2) 

Notch同源物2(NOTCH2)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues:  Ala2251~Asn2466

Tags: N-terminal His Tag

Subcellular Location: Nucleus

Purity: > 97%

Traits: Freeze-dried powder

Buffer formulation: 100mMNaHCO₃, 500mMNaCl, pH8.3, containing 1mM DTT, 0.01% SKL,5% Trehalose and Proclin300.

Original Concentration: 200µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 6.3

Predicted Molecular Mass:  26.6kDa

Accurate Molecular Mass:  33kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in 100mM NaHCO₃, 500mM NaCl (pH8.3) to a concentration of 0.1-1.0 mg/mL. Donot vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8ºC for one month.

             Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

[  IDENTIFICATION  ]

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