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SIRT7抗原;沉默调节蛋白7重组蛋白多种属

SIRT7抗原;沉默调节蛋白7重组蛋白多种属 SIRT7是sirtuin蛋白家族的成员,与酵母Sir2蛋白同源。sirtuin家族的成员以sirtuin核心结构域为特征,分为四类。人类去乙酰化酶的功能尚未确定;然而,酵母去乙酰化酶蛋白可以调节表观遗传基因沉默并抑制rDNA的重组。研究表明,人去乙酰化酶可能作为具有单ADP核糖基转移酶活性的细胞内调节蛋白发挥作用。该基因编码的蛋白质属于si

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  • 更新时间:2024-12-17
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详细介绍
品牌上海信裕生物科技有限公司CAS详见说明书
分子式详见说明书纯度> 90%
分子量48.6kDa货号XY94647RPHu01
规格50µg/100µg供货周期一周
主要用途SDS-PAGE; WB应用领域生物产业

Recombinant Sirtuin 7 (SIRT7)  

沉默调节蛋白7(SIRT7)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues:  Met1~Thr400

Tags: N-terminal His Tag

Subcellular Location: Nucleus, Cytoplasm

Purity: > 90%

Traits: Freeze-dried powder

Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose.

Original Concentration: 50µg/mL

Applications: Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.8

Predicted Molecular Mass:  48.6kDa

Accurate Molecular Mass:  44/48kDa as determined by SDS-PAGE reducing conditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in ddH2O to a concentration of 0.1-0.4 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8ºC for one month.

           Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

SIRT7抗原;沉默调节蛋白7重组蛋白多种属

[  IDENTIFICATION  ]

SIRT7抗原;沉默调节蛋白7重组蛋白多种属

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!


























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