Articles
UBE3A抗原;泛素连接酶E3A重组蛋白多种属 E6结合蛋白是泛素介导的蛋白水解途径的一个组成部分,该途径选择性地靶向26S蛋白酶体降解的蛋白质。泛素(Ub)通过将Ub从E2泛素结合酶转移到靶蛋白,直接与蛋白质底物结合。E3泛素连接酶家族成员如E6-AP的酶活性促进了这种结合。E6-AP的几种底物已被鉴定,包括肿瘤抑制蛋白p53和Rad23的哺乳动物同源物HHR23A。先前的研究表明,E6-AP与
联系电话:15221858802
品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 90% |
分子量 | 32.0kDa | 货号 | XY95238RPHu |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant Ubiquitin Protein Ligase E3A (UBE3A)
泛素连接酶E3A(UBE3A)重组蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Ser158~Leu407
Tags: N-terminal His Tag
Subcellular Location: Nucleus
Purity: > 90%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 500µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 6.1
Predicted Molecular Mass: 32.0kDa
Accurate Molecular Mass: 40kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!
电话