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HMGN2抗原;HMGN2重组蛋白多种属 高迁移率族(HMG)蛋白14和17是丰富的染色体蛋白,与核小体结合并增强转录(1-5)。HMG-14和HMG-17也作为结构元件发挥作用,改变染色质纤维的结构,增强染色质模板的转录(1-3,5)。HMG-14/17蛋白修饰30 nm染色质纤维的核小体组织,并介导高阶染色质结构的展开,从而促进对底层DNA序列的访问(1-3)。将HMG蛋白和接头组蛋白亚型等结
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 90% |
分子量 | 12.1kDa | 货号 | XY92419RPHu |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant High Mobility Group Nucleosome Binding Domain Containing Protein 2 (HMGN2)
高迁移率族核小体结合的结构域2(HMGN2)重组蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Pro2~Gln81
Tags: N-terminal His Tag
Subcellular Location: Cytoplasm, Chromosome
Purity: > 90%
Traits: Freeze-dried powder
Buffer formulation:PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 500µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 10.2
Predicted Molecular Mass: 12.1kDa
Accurate Molecular Mass: 17kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!
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