Articles
RRBP1抗原;核糖体结合蛋白1重组蛋白多种属 RRBP1(核糖体结合蛋白1)作为核糖体受体,介导核糖体和内质网膜之间的相互作用。
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 95% |
分子量 | 21.1kDa | 货号 | XY94442RPHu |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB; ELISA; IP. | 应用领域 | 生物产业 |
Recombinant Ribosome Binding Protein 1 (RRBP1)
核糖体结合蛋白1(RRBP1)重组蛋白
[ PROPERTIES ]
Residues: Arg822~Val977
Tags: Two N-terminal Tags, His-tag and T7-tag
Accession: Q9P2E9
Host: E. coli
Subcellular Location: Endoplasmic reticulummembrane; Single-pass type III membrane protein.
Purity: >95%
Endotoxin Level: <1.0EU per 1μg
(determined by the LAL method).
Formulation: Supplied as lyophilized form in 20mM Tris,150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% sarcosyl, 5% trehalose, and preservative.
Predicted isoelectric point: 4.7
Predicted Molecular Mass: 21.1kDa
Accurate Molecular Mass: 30kDa as determined by SDS-PAGE reducingconditions.
Applications: SDS-PAGE; WB; ELISA; IP.
(May be suitable for use in other assays to be determined by the end user.)
Note: The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in sterile ddH2O.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
The sequence of the target protein is listed below.
RQQLSEMKS HVEDGDIAGA PASSPEAPPA EQDPVQLKTQ LEWTEAILED EQTQRQKLTAEFEEAQTSAC RLQEELEKLR TAGPLESSET EEASQLKERL EKEKKLTSDL GRAATRLQELLKTTQEQLAR EKDTVKKLQE QLEKAEDGSS SKEGTSV
[ IDENTIFICATION ]
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