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转化生长因子β诱导蛋白(TGFbI)重组蛋白 该基因编码一种含RGD的蛋白质,该蛋白质与I、II和IV型胶原蛋白结合。RGD基序存在于许多调节细胞粘附的细胞外基质蛋白中,并作为几种整合素的配体识别序列。这种蛋白质在细胞-胶原相互作用中起作用,可能参与软骨内骨的形成。该蛋白由转化生长因子β诱导,并起到抑制细胞粘附的作用。该基因的突变与多种类型的角膜营养不良有关。【由RefSeq提供,2008年7月】
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 95% |
分子量 | 26.8kDa | 货号 | XY95066RPHu01 |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB; ELISA; IP; CoIP; Purificat | 应用领域 | 生物产业 |
Recombinant Transforming Growth Factor Beta Induced Protein (TGFbI)
转化生长因子β诱导蛋白(TGFbI)重组蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Gly423~Leu632
Tags: N-terminal His Tag
Tissue Specificity: Brain, Heart, Liver, Kidney.
Subcellular Location: Secreted, extracellular space, extracellular matrix
Purity: > 95%
Traits: Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.
Original Concentration: 200µg/mL
Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 9.4
Predicted Molecular Mass: 26.8kDa
Accurate Molecular Mass: 31kDa as determined by SDS-PAGE reducingconditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
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