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转化生长因子β诱导蛋白(TGFbI)重组蛋白

转化生长因子β诱导蛋白(TGFbI)重组蛋白 该基因编码一种含RGD的蛋白质,该蛋白质与I、II和IV型胶原蛋白结合。RGD基序存在于许多调节细胞粘附的细胞外基质蛋白中,并作为几种整合素的配体识别序列。这种蛋白质在细胞-胶原相互作用中起作用,可能参与软骨内骨的形成。该蛋白由转化生长因子β诱导,并起到抑制细胞粘附的作用。该基因的突变与多种类型的角膜营养不良有关。【由RefSeq提供,2008年7月】

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详细介绍
品牌上海信裕生物科技有限公司CAS详见说明书
分子式详见说明书纯度> 95%
分子量26.8kDa货号XY95066RPHu01
规格50µg/100µg供货周期一周
主要用途SDS-PAGE; WB; ELISA; IP; CoIP; Purificat应用领域生物产业

Recombinant Transforming Growth Factor Beta Induced Protein (TGFbI) 

转化生长因子β诱导蛋白(TGFbI)重组蛋白

[ PROPERTIES ]

Source: Prokaryotic expression

Host: E.coli

Residues:  Gly423~Leu632

Tags: N-terminal His Tag

Tissue Specificity: Brain, Heart, Liver, Kidney.

Subcellular Location: Secreted, extracellular space, extracellular matrix

Purity: > 95%

Traits: Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.

Original Concentration: 200µg/mL

Applications: SDS-PAGE; WB; ELISA; IP; CoIP; Purification; Amine ReactiveLabeling.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point: 9.4

Predicted Molecular Mass:  26.8kDa

Accurate Molecular Mass:  31kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8ºC for one month.

           Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

转化生长因子β诱导蛋白(TGFbI)重组蛋白

[  IDENTIFICATION  ]

转化生长因子β诱导蛋白(TGFbI)重组蛋白

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