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赖氨酰氧化酶样蛋白1(LOXL1)重组蛋白

赖氨酰氧化酶样蛋白1(LOXL1)重组蛋白 该基因编码赖氨酰氧化酶基因家族的成员。该家族的原型成员对结缔组织的生物合成至关重要,编码一种细胞外铜依赖性胺氧化酶,催化胶原蛋白和弹性蛋白交联形成的第一步。C末端高度保守的氨基酸序列似乎足以维持胺氧化酶的活性,这表明每个家族成员都可能保留这一功能。N-末端保守度很低,可能会在发育调节、衰老、肿瘤抑制、细胞生长控制和趋化性方面为家族的每个成员赋予额外的作用

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  • 更新时间:2025-01-10
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详细介绍
品牌上海信裕生物科技有限公司CAS详见说明书
分子式详见说明书纯度> 92%
分子量24.8kDa货号XY93055RPHu01
规格50µg/100µg供货周期一周
主要用途SDS-PAGE; WB应用领域生物产业

Recombinant Lysyl Oxidase Like Protein 1 (LOXL1) 

赖氨酰氧化酶样蛋白1(LOXL1)重组蛋白

[ PROPERTIES ]

Source:Prokaryotic expression

Host: E.coli

Residues: Leu369~Ser574

Tags:N-terminal His Tag

Tissue Specificity:Placenta, Lung.

Subcellular Location: Secreted, extracellular space.

Purity: > 92%

Traits:Freeze-dried powder

Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.

Original Concentration:200µg/mL

Applications:Positive Control; Immunogen; SDS-PAGE; WB.

(May be suitable for use in other assays to be determined by the end user.)

Predicted isoelectric point:6.3

Predicted Molecular Mass: 24.8kDa

Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducingconditions.

Phenomenon explanation:

The possible reasons that the actual band size differs from the predicted are as follows:

1.Splice variants: Alternative splicing may create different sized proteins from the same gene.

2. Relative charge: The composition of amino acids may affects the charge of the protein.

3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.

4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.

5. Polymerization of the target protein: Dimerization, multimerization etc.

[ USAGE ]

Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8ºC for one month.

           Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

赖氨酰氧化酶样蛋白1(LOXL1)重组蛋白

[  IDENTIFICATION  ]

赖氨酰氧化酶样蛋白1(LOXL1)重组蛋白

[ IMPORTANT NOTE ]

The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.

特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!




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