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赖氨酰氧化酶样蛋白1(LOXL1)重组蛋白 该基因编码赖氨酰氧化酶基因家族的成员。该家族的原型成员对结缔组织的生物合成至关重要,编码一种细胞外铜依赖性胺氧化酶,催化胶原蛋白和弹性蛋白交联形成的第一步。C末端高度保守的氨基酸序列似乎足以维持胺氧化酶的活性,这表明每个家族成员都可能保留这一功能。N-末端保守度很低,可能会在发育调节、衰老、肿瘤抑制、细胞生长控制和趋化性方面为家族的每个成员赋予额外的作用
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 92% |
分子量 | 24.8kDa | 货号 | XY93055RPHu01 |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant Lysyl Oxidase Like Protein 1 (LOXL1)
赖氨酰氧化酶样蛋白1(LOXL1)重组蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues: Leu369~Ser574
Tags:N-terminal His Tag
Tissue Specificity:Placenta, Lung.
Subcellular Location: Secreted, extracellular space.
Purity: > 92%
Traits:Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 1mM DTT, 5%trehalose, 0.01%sarcosyl and Proclin300.
Original Concentration:200µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:6.3
Predicted Molecular Mass: 24.8kDa
Accurate Molecular Mass: 28kDa as determined by SDS-PAGE reducingconditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!
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