原始广泛存在蛋白1(AUP1)重组蛋白

Recombinant Ancient Ubiquitous Protein 1 (AUP1)  

原始广泛存在蛋白1(AUP1)重组蛋白

[ PROPERTIES ]

Residues: Val201~Asp410

Tags: Two N-terminal Tags, His-tag and T7-tag

Accession: Q9Y679

Host:E. coli

Subcellular Location: Endoplasmic reticulum, Membrane.

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% trehalose, 0.01% sarcosyl.

Predicted isoelectric point: 9.7

Predicted Molecular Mass: 27.2kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8ºC for one month.

           Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The sequence of the target protein is listed below.

VSDASWVSEL LWSLFVPFTV YQVRWLRPVH RQLGEANEEF ALRVQQLVAK ELGQTGTRLTPADKAEHMKR QRHPRLRPQS AQSSFPPSPG PSPDVQLATL AQRVKEVLPHVPLGVIQRDLAKTGCVDLTI TNLLEGAVAF MPEDITKGTQ SLPTASASKF PSSGPVTPQP TALTFAKSSWARQESLQERK QALYEYARRR FTERRAQEAD

[  IDENTIFICATION  ]

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