LDHA抗原;乳酸脱氢酶A(LDHA)重组蛋白多种属 该基因编码的蛋白质在厌氧糖酵解的最后一步催化L-乳酸和NAD转化为丙酮酸和NADH。该蛋白质主要存在于肌肉组织中,属于乳酸脱氢酶家族。该基因的突变与劳力性肌红蛋白尿有关。已发现该基因编码不同亚型的多种转录变体。人类基因组包含该基因的几个非转录假基因。【由RefSeq提供】。
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 95% |
分子量 | 40.8kDa | 货号 | XY92882RPHu01 |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant Lactate Dehydrogenase A (LDHA)
乳酸脱氢酶A(LDHA)重组蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Met1~Phe332 plus VPVC between Ser197 and Val198
Tags: N-terminal His Tag
Subcellular Location: Cytoplasm
Purity: > 95%
Traits: Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 1mM EDTA, 1mM DTT,0.01% SKL, 5% Trehalose and Proclin300.
Original Concentration: 200µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point: 8.1
Predicted Molecular Mass: 40.8kDa
Accurate Molecular Mass: 33kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 20mM Tris, 150mM NaCl (pH8.0) to a concentration of 0.1-1.0 mg/mL. Do notvortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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