半胱氨酸蛋白酶抑制剂A(CSTA)重组蛋白

Recombinant Cystatin A (CSTA) 

半胱氨酸蛋白酶抑制剂A(CSTA)重组蛋白

[ PROPERTIES ]

Residues: Met1~Phe98 , withN-terminal His-Tag.

Host: E. coli

Subcellular Location: Cytoplasm. Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation: Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 6.4

Predicted Molecular Mass: 12.2kDa

Applications: SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

             Store at 2-8ºC for one month.

             Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with N-terminal His-Tag, its sequence is listedbelow. MGHHHHHHSGS-MIPGGLSEAK PATPEIQEIV DKVKPQLEEK TNETYGKLEA VQYKTQVVAGTNYYIKVRAG DNKYMHLKVF KSLPGQNEDL VLTGYQVDKN KDDELTGF

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