Articles
LRRFIP1抗原;LRRFIP1重组蛋白多种属 LRRFIP1是一种738个氨基酸的人类蛋白质,其啮齿动物对应物被称为LRRFIP1(在小鼠中也称为FLAP)。LRRFIP1也被认为通过PDGF-A抑制来控制动脉损伤后平滑肌细胞的增殖。LRRFIP1的N端与FLAP的卷曲螺旋结构域显示出高度同源性,FLAP是一种结合Flightless I的富含亮氨酸重复序列(LRR)的蛋白质,LRRFIP1与
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 90% |
分子量 | 14.2kDa | 货号 | XY92937RPHu01 |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant Leucine Rich Repeat In FLII Interacting Protein 1 (LRRFIP1)
亮氨酸丰富重复FLII相互作用蛋白1(LRRFIP1)重组蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues: Leu615~Asp735
Tags:N-terminal His Tag
Subcellular Location: Membrane, Cytoplasm
Purity: > 90%
Traits:Freeze-dried powder
Buffer formulation: PBS, pH7.4, containing 0.01% SKL, 5% Trehalose.
Original Concentration:200µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:4.37
Predicted Molecular Mass: 14.2kDa
Accurate Molecular Mass: 20kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in 10mM PBS (pH7.4) to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!
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