Articles
CA125抗原;糖抗原125重组蛋白多种属 CA125是与卵巢癌细胞相关的膜表面糖蛋白。在正常情况下卵巢细胞中无CA125抗原,但在经期和孕期妇女的卵巢中发现存在由此种抗原。
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 95% |
分子量 | 22.2kDa | 货号 | XY90764RPHu01 |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant Carbohydrate Antigen 125 (CA125)
糖抗原125(CA125)重组蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues:Arg13977~Ser14117
Tags:N-terminal His Tag
Subcellular Location: Membrane, Secreted
Purity: > 95%
Traits:Freeze-dried powder
Buffer formulation: 100mMNaHCO3, 500mMNaCl, pH8.3, containing 0.01% SKL, 5%Trehalose.
Original Concentration:150µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:6.2
Predicted Molecular Mass:22.2kDa
Accurate Molecular Mass:26kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0.1-0.2 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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