SPINK5抗原；SPINK5重组蛋白多种属

Recombinant Serine Peptidase Inhibitor Kazal Type 5 (SPINK5)  

丝氨酸肽酶抑制因子Kazal型5(SPINK5)重组蛋白

[ PROPERTIES ]

Residues: Gly699~Ser976,with two N-terminal Tags, His-tag and T7-tag.

Host:E. coli

Subcellular Location: Secreted

Purity: >95%

Endotoxin Level: <1.0EU per 1μg

(determined by the LAL method).

Formulation:Supplied as lyophilized form in PBS,pH7.4, containing 5% sucrose, 0.01% sarcosyl.

Predicted isoelectric point: 8.8

Predicted Molecular Mass: 35.7kDa

Applications:SDS-PAGE; WB; ELISA; IP.

(May be suitable for use in other assays to be determined by the end user.)

[ USAGE ]

Reconstitute in sterile PBS, pH7.2-pH7.4.

[ STORAGE AND STABILITY ]

Storage: Avoid repeated freeze/thaw cycles.

           Store at 2-8ºC for one month.

           Aliquot and store at -80ºC for 12 months.

Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.

[SEQUENCE ]

The target protein is fused with two N-terminal Tags, His-tag and T7-tag, itssequence is listed below.

MGSS HH HHH H SSG LVP RGS H MASMTG GQ QM GRGSEF -G G GNTQDE CA EYREQMK NG R LS CT RE S DPV RD ADGKSY N NQ C TM CKA K LE RE AE RK NEYS RSRS NG TGSESG KDT CDEF RS Q MK N GK LI CT R ES DPV RGP DG KT HG NK C TMCKEKLEREAAE KKKKEDEDRS NTGERSNTGE RSNDKEDLCR EFRSMQRNGK LICTRENNPVRGPYGKMHIN KCAMCQSIFD REANERKKKD EEKSSSKPSN NAKDECSEFRNYIRNNELICPRENDPVHGA DGKFYTNKCY MCRAVFLTEA LERAKLQEKP SHVRAS

[  IDENTIFICATION  ]

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