Articles
IRF1抗原;干扰素调节因子1重组蛋白多种属 IRF1编码干扰素调节因子1,它是干扰素调节转录因子(IRF)家族的成员。IRF1是干扰素α和β转录的激活因子,在小鼠中,它已被证明是这些基因双链RNA诱导所必需的。IRF1还充当干扰素α、β和γ诱导的基因的转录激活剂。此外,IRF1已被证明在调节细胞凋亡和肿瘤抑制中发挥作用。
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 90% |
分子量 | 31.2kDa | 货号 | XY92663RPHu01 |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant Interferon Regulatory Factor 1 (IRF1)
干扰素调节因子1(IRF1)重组蛋白
[ PROPERTIES ]
Source: Prokaryotic expression
Host: E.coli
Residues: Trp11~Glu276
Tags: N-terminal His Tag
Subcellular Location: Nucleus, Cytoplasm
Purity: > 90%
Traits: Freeze-dried powder
Buffer formulation:20mM Tris, 150mM NaCl, pH8.0, containing 0.01% SKL, 5% Trehalose.
Original Concentration: 50µg/mL
Applications: Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:5.7
Predicted Molecular Mass:31.2kDa
Accurate Molecular Mass: 39kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0-0.5 mg/mL. Do not vortex..
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
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