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丝氨酸羟甲基转移酶2(SHMT2)重组蛋白 该基因编码磷酸吡哆醛依赖性酶的线粒体形式,该酶催化丝氨酸和四氢叶酸转化为甘氨酸和5,10-亚甲基四氢叶酸的可逆反应。编码产物主要负责甘氨酸的合成。编码蛋白的活性被认为是细胞内甘氨酸的主要来源。编码这种酶细胞质形式的基因位于17号染色体上。选择性剪接导致多种转录变体。【由RefSeq提供,2009年10月】
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品牌 | 上海信裕生物科技有限公司 | CAS | 详见说明书 |
---|---|---|---|
分子式 | 详见说明书 | 纯度 | > 97% |
分子量 | 56.3KDa | 货号 | XY94549RPHu |
规格 | 50µg/100µg | 供货周期 | 一周 |
主要用途 | SDS-PAGE; WB | 应用领域 | 生物产业 |
Recombinant Serine Hydroxymethyltransferase 2, Mitochondrial (SHMT2)
丝氨酸羟甲基转移酶2(SHMT2)重组蛋白
[ PROPERTIES ]
Source:Prokaryotic expression
Host: E.coli
Residues: Asn30~His504
Tags:N-terminal His Tag
Subcellular Location: Secreted
Purity: > 97%
Traits:Freeze-dried powder
Buffer formulation: 20mM Tris, 150mM NaCl, pH8.0, containing 0.01% skl, 5%Trehalose.
Original Concentration:100µg/mL
Applications:Positive Control; Immunogen; SDS-PAGE; WB.
(May be suitable for use in other assays to be determined by the end user.)
Predicted isoelectric point:8.1
Predicted Molecular Mass: 56.3KDa
Accurate Molecular Mass: 60kDa as determined by SDS-PAGE reducing conditions.
Phenomenon explanation:
The possible reasons that the actual band size differs from the predicted are as follows:
1.Splice variants: Alternative splicing may create different sized proteins from the same gene.
2. Relative charge: The composition of amino acids may affects the charge of the protein.
3. Post-translational modification: Phosphorylation, glycosylation, methylation etc.
4. Post-translation cleavage: Many proteins are synthesized as pro-proteins, and then cleavedto give the active form.
5. Polymerization of the target protein: Dimerization, multimerization etc.
[ USAGE ]
Reconstitute in ddH2O to a concentration of 0.1-1.0 mg/mL. Do not vortex.
[ STORAGE AND STABILITY ]
Storage: Avoid repeated freeze/thaw cycles.
Store at 2-8ºC for one month.
Aliquot and store at -80ºC for 12 months.
Stability Test: The thermal stability is described by the loss rate. The loss rate was determined by accelerated thermal degradation test, that is, incubate the protein at 37°C for 48h, and no obvious degradation and precipitation were observed. The loss rate is less than 5% within the expiration date under appropriate storage condition.
[SEQUENCE ]
[ IDENTIFICATION ]
[ IMPORTANT NOTE ]
The kit is designed for research use only, we will not be responsible for any issue if the kit was used in clinical diagnostic or any other procedures.
特别提示:本公司的所有产品仅可用于科研实验,严禁用于临床医疗及其他非科研用途!以实际收货产品说明书为准,网站说明书仅供参考!
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